Application

Anti-Integrin beta-1, clone 102DF5, Cat. No. MABT1502, is a mouse monoclonal antibody that detects Integrin beta-1 and has been tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Flow Cytometry Analysis: A representative lot detected Integrin beta-1 in Flow Cytometry applications (Belkin, A.M., et. al. (1997). J Cell Biol. 139(6):1583-95).

Immunofluorescence Analysis: A representative lot detected Integrin beta-1 in Immunofluorescence applications (Cartier-Michaud, A., et. al. (2012). PLoS One. 7(2):e32204; Balzac, F., et. al. (1993). J Cell Biol. 121(1):171-8; Lin, Y.N., et. al. (2015). Oncotarget. 6(21):18577-89).

Western Blotting Analysis: A representative lot detected Integrin beta-1 in Western Blotting applications (Balzac, F., et. al. (1993). J Cell Biol. 121(1):171-8).

ELISA Analysis: A representative lot detected Integrin beta-1 in ELISA applications (Jovanovic, M., et. al. (2010). Act Histochem. 112(1):34-41).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin beta-1 in Immunoprecipitation applications (Balzac, F., et. al. (1993). J Cell Biol. 121(1):171-8).

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin beta-1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin beta-1 is a single-pass type I membrane protein that can associate with one of the multiple alpha subunits to serve as a receptor for fibronectin and other extracellular matrix proteins. Integrin beta-1 integrin recognizes the sequence R-G-D in a wide array of ligands. Five isoforms of integrin beta-1 have been reported that are produced by alternative splicing. Integrin beta-1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). Isoform 1 of integrin beta-1 is widely expressed and other isoforms are generally co-expressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4 is selectively expressed in peripheral T-cells and isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). Isoform 2 is reported to interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).

Immunogen

Tissue extract from human myometrium.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry (Paraffin) in human uterus and human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Integrin beta-1 in human uterus and human kidney tissue sections.

Specificity

Clone 102DF5 specifically detects integrin beta-1 subunit in human cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed; 88.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).